AbstractThis chapter discusses the applications of arbitrarily amplified DNA (AAD) in plant genotyping, such as in nucleic acid scanning. The optimization and reproducibility of the technique are covered, and the challenges and limitations of AAD markers are described in applications related to turfgrass science (i.e. characterization of turfgrass species, cultivars and mutations) and floriculture (i.e. distinguishing closely related species of ornamental flowering plants) are mentioned.