Author:
Bruchem J. Van,Voight J,Lammers-Wienhoven Toos S. C. W,Schönhusen Ulrike,Ketelaars J. J. M. H,Tamminga S
Abstract
Sheep fitted with a PVC cannula in the dorsal rumen, a silastic infusion tube in the abomasum, and single ‘T’-shape PVC cannulas in the proximal duodenum (D1), distal duodenum (D2), mid-jejunum (J), and terminal ileum (I), were fed on diets varying in crude protein (Nx6·25) and fibre contents.15N was administered to the sheep as a15N-labelled grass meal-beer yeast suspension which was continuously infused into the abomasum over a period of 1 week. Subsequently, over a period of 4 d,15N excess in the D1, D2, J and I digesta and faeces was measured and related to15N excess in blood plasma TCA-soluble N. Average daily intakes of DM and N were 1·12 kg and 26·3 g respectively. Average daily endogenous N (g) amounted to 3·1 at D1, 10·2 at D2, 6·1 at J, 5·0 at I, and 5·1 in the faeces (F). Corresponding percentage values for total N were: D112·4, D232·9, J 46-4, I 48.4 and F 50.7. The secretion, particularly of pancreatic juice and/or bile, varied. The apparent re-absorption of endogenous protein varied among experimental diets, on average by 50·4% between D2and I. Along the small intestine the percentage of15N in the TCA-precipitable fraction gradually changed, on average by 66·0, 63·0, 46·3 and 55·7 at D1, D2, J and I respectively. Because of differential rates of enrichment and turnover among protein pools which contribute towards endogenous secretion, care should be taken in interpreting these data as absolute values.
Publisher
Cambridge University Press (CUP)
Subject
Nutrition and Dietetics,Medicine (miscellaneous)
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