Molecular genetic characterization of members of the genus Toxocara - taxonomic, population genetic and epidemiological considerations.

Abstract

This chapter gives an account of molecular methods for the specific identification of species of Toxocara and their differentiation from other ascaridids with zoonotic potential, for the diagnosis of infections and for the analysis of genetic variation within and among individual nematodes and their populations. This chapter emphasizes the utility of polymerase chain reaction-based tools for studying the taxonomy of members within the genus Toxocara and for investigating their population genetics, ecology and epidemiology. It is concluded that molecular methods provide a platform for tackling diagnostic problems and biological questions relating to species of Toxocara, provided careful consideration is given to sampling procedures, genetic markers and analytical approaches employed.