Alkaloid profiles of Mimosa tenuiflora and associated methods of analysis.

Author:

Gardner D. R.,Riet-Correa F.,Panter K. E.

Abstract

Abstract

The alkaloid contents of the leaves and seeds of M. tenuiflora collected from northeastern Brazil were studied. Alkaloids were isolated by classical acid/base extraction procedures and by cation exchange solid phase extraction. The crude alkaloid fractions were then analysed by thin layer chromatography (TLC), gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-mass spectrometry (LC-MS). In the initial analysis by GC-MS, two major alkaloids were detected and were identified as N,N-dimethyltryptamine (DMT) and 2-methylcarboline. A third alkaloid was only detected when extraction by method A was conducted. TLC analysis of the extracts also showed the presence of two major alkaloids. DMT and 2-methylcarboline were detected following GC-MS analysis. LC/UV/MS detected five alkaloids with UV and MS data consistent with tryptamine type alkaloids. These included DMT and 2-methylcarboline, and three unknown alkaloids. At least three possible minor alkaloids were detected, but their UV spectra was not consistent with the tryptamine type alkaloids. The presence of a possible artifact alkaloid was observed by LC-UV-MS analysis when extracts were prepared using extraction method A. Seed and leaf samples collected at different years were extracted and analysed by TLC and LC-MS. Significant differences were observed in the alkaloid profiles between seed and leaves in that the two major spots in the TLC chromatograms were absent in seed samples. The best method of analysis appeared to be solid phase extraction followed by LC/UV/MS.

Publisher

CABI

Cited by 6 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3