Comparison of Incubation at 30 and 32 C for 48 and 72 Hours for Enumeration of Raw-Milk Bacteria1

Author:

HUHTANEN C. N.,BRAZIS A. R.,ANDERSON H. J.,ARLEDGE W. L.,DONNELLY C. B.,GINN R. E.,KOCH E. J.,NELSON F. E.,LaGRANGE W. S.,PETERSON D. E.,RANDOLPH H. E.,SING E. L.,THOMPSON D. I.,WEHR H. M.

Abstract

Plates incubated for 48 h showed significantly higher (0.01 <P<0.05) counts at 30 than 32 C (arithmetic means were 5.4% higher; geometric means were 1.5% higher). These higher counts, however, were largely obtained by two of 15 analysts representing 12 of 135 samples. Seventy-two-hour incubation gave significantly higher (P<0.01) counts than 48 h (arithmetic means for 72 h were 4.53% higher than 48 h; geometric means for 72 h were 2.58% higher) at both temperatures. There were interaction effects indicating a geographical or personal bias in the results.

Publisher

International Association for Food Protection

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