Phenotypic and Genotypic Characterization of Salmonella Isolates Recovered from Foods Linked to Human Salmonellosis Outbreaks in Minas Gerais State, Brazil-Reference-Cited by-同舟云学术

Phenotypic and Genotypic Characterization of Salmonella Isolates Recovered from Foods Linked to Human Salmonellosis Outbreaks in Minas Gerais State, Brazil

Published:2021-09-15 Issue:1 Volume:85 Page:142-154
ISSN:0362-028X
Container-title:Journal of Food Protection
language:en
Short-container-title:

Author:

FAULA LEANDRO L.¹²^ORCID,CERQUEIRA MONICA M. O. P.²,MADEIRA JOVITA E. G. C.¹,SOUZA MARCELO R.²,SOUZA FERNANDO N.³⁴,AMANCIO GLÁUCIA C. S.¹

Affiliation:

1. Divisão de Vigilância Sanitária e Ambiental, Laboratório Central de Saúde Pública de Minas Gerais, Fundação Ezequiel Dias, Rua Conde Pereira Carneiro 80, Belo Horizonte 30510-010, Brazil

2. Departamento de Tecnologia e Inspeção de Produtos de Origem Animal, Escola de Medicina Veterinária, Universidade Federal de Minas Gerais, Av. Presidente Antônio Carlos 6627, Belo Horizonte 31270-901, Brazil

3. Departamento de Medicina Interna, Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Av. Prof. Dr. Orlando Marques de Paiva 87, São Paulo 05508-270, Brazil

4. Programa de Pós Graduação em Ciência Animal, Universidade Federal da Paraíba, Areia 58397-000, Brazil

Abstract

ABSTRACT Salmonella is one of the primary pathogens that causes foodborne diseases worldwide. In the present study, we characterized Salmonella isolates recovered from foods linked to human salmonellosis outbreaks in Minas Gerais, Brazil, from 2003 to 2017. Serotype, antimicrobial susceptibility, presence of virulence genes, and genetic polymorphism as determined by repetitive element sequence–based PCR were determined for 70 Salmonella isolates. Thirteen Salmonella serotypes were identified, and the most prevalent were Enteritidis and Typhimurium, comprising 52 (74.3%) of the 70 isolates. Sixty-five (92.8%) of the isolates were resistant to at least 1 of the 15 antimicrobials tested. Ten isolates (14.2%) had a multidrug resistance phenotype. Isolates were screened for 16 virulence genes, which were found in 75.7 to 100% of the isolates. A statistical difference was found among Salmonella serotypes in the presence of the sipB, sopE, lfpA, sefA, and spvC genes. Based on their DNA fingerprints, 40 isolates of Salmonella Enteritidis from 16 outbreaks were separated into 14 groups and 12 isolates of Salmonella Typhimurium were separated into 6 groups. These serological patterns were similar to those reported by public health centers worldwide. Of concern is the high prevalence among the isolates in this study of both virulence genes and resistance to antimicrobials, especially to critically important drugs. Special attention should be given to Salmonella Enteritidis. Although the genomes of these Salmonella isolates were relatively variable, high genetic similarity was observed among them, and some had identical fingerprints. These results support the hypothesis of clonal circulation of Salmonella isolates causing human infections in Minas Gerais. HIGHLIGHTS

Publisher

International Association for Food Protection

Subject

Microbiology,Food Science

Link

https://meridian.allenpress.com/jfp/article-pdf/85/1/142/3001704/i0362-028x-85-1-142.pdf

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