Recommended Assay for Treatment of Chicks to Prevent Salmonella Colonization by ‘Competitive Exclusion’

Author:

MEAD GEOFFREY C.1,BARROW PAUL A.2,HINTON MICHAEL H.3,HUMBERT FLORENCE4,IMPEY CLIVE S.1,LAHELLEC CECILE4,MULDER ROEL W. A. W.5,STAVRIC STANKA6,STERN NORMAN J.7

Affiliation:

1. 1Agricultural and Food Research Council Institute of Food Research, Bristol Laboratory, Langford, Bristol BS18 7DY, UK

2. 2Agricultural and Food Research Council Institute for Animal Health, Houghton Laboratory, Houghton, Huntingdon PE17 2DA, Cambs., UK

3. 3School of Veterinary Science, Langford House, Langford, Bristol BS18 7DU, UK

4. 4Centre National d'Etudes Vétérinaires et Alimentaires, Station Experimentale d'Aviculture, BP No 9, Beaucemaine, 22440 Ploufragan, France

5. 5Spelderholt Centre for Poultry Research and Extension, Spelderholt 9, 7361 DA Beekbergen, The Netherlands

6. 6Health and Welfare Canada, Bureau of Microbial Hazards, Sir Frederick Banting Research Centre, Ottawa, Ontario K1A OLZ, Canada

7. 7United States Department of Agriculture, Agricultural Research Service, Richard B. Russell Agricultural Research Center, P.O. Box 5677, Athens, Georgia 30613, USA

Abstract

An assay is described for evaluating live-culture treatment material that may be given orally to chicks to prevent intestinal colonization by non-host-specific salmonellae. Both pre-treated and control chicks are challenged with ca 104 salmonellae/chick, using a strain bearing an antibiotic resistance marker. Chicks are examined 5 d after challenge to determine both the proportion of positive birds in treated and control groups and the levels of Salmonella in the caeca of infected individuals. The efficacy of the treatment is determined by calculation of values for Infection Factor and Protection Factor.

Publisher

International Association for Food Protection

Subject

Microbiology,Food Science

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