Multilaboratory Validation of a Luminex Microbead-Based Suspension Array for the Identification of the 11 Most Clinically Relevant Shiga Toxin–Producing Escherichia coli O Serogroups†-Reference-Cited by-同舟云学术

Multilaboratory Validation of a Luminex Microbead-Based Suspension Array for the Identification of the 11 Most Clinically Relevant Shiga Toxin–Producing Escherichia coli O Serogroups†

Published:2013-05-01 Issue:5 Volume:76 Page:867-870
ISSN:0362-028X
Container-title:Journal of Food Protection
language:en
Short-container-title:

Author:

LIN ANDREW¹,KASE JULIE A.²,MOORE MICHELLE M.³,SON INSOOK²,TRAN NELLY⁴,CLOTILDE LAURIE M.¹,JARVIS KAREN⁵,JONES KELLY⁶,KASTURI KUPPUSWAMY⁷,NABE KHAMPHET⁸,NUCCI MELISSA⁹,WAGLEY GAIL S.¹⁰,WANG FEI⁶,GE BEILEI⁶,HAMMACK THOMAS S.²

Affiliation:

1. 1U.S. Food and Drug Administration, San Francisco District, Alameda, California 94502

2. 2U.S. Food and Drug Administration, Center for Food Safety and Applied Nutrition, Office of Regulatory Science, College Park, Maryland 20740

3. 3U.S. Food and Drug Administration, Pacific Regional Laboratory Northwest, Applied Technology Center, Bothell, Washington 98021

4. 4U.S. Food and Drug Administration, Pacific Regional Laboratory Southwest, Irvine, California 92612

5. 5U.S. Food and Drug Administration, Center for Food Safety and Applied Nutrition, Office of Applied Research and Safety Assessments, Laurel, Maryland 20708

6. 6U.S. Food and Drug Administration, Center for Veterinary Medicine, Office of Research, Laurel, Maryland 20708

7. 7U.S. Food and Drug Administration, Northeast Regional Laboratory, Jamaica, New York 11433

8. 8U.S. Food and Drug Administration, Pacific Regional Laboratory Northwest, Bothell, Washington 98021

9. 9U.S. Food and Drug Administration, Denver District Laboratory, Lakewood, Colorado 80225

10. 10U.S. Food and Drug Administration, Southeast Regional Laboratory, Atlanta, Georgia 30309, USA

Abstract

Rapid and high-throughput identification and serotyping of Shiga toxin–producing Escherichia coli (STEC) O serogroups is important for detecting, investigating, and controlling STEC infection outbreaks and removing hazardous products from commerce. A Luminex microbead-based suspension array has been developed to identify the 11 most clinically relevant STEC serogroups: O26, O45, O91, O103, O104, O111, O113, O121, O128, O145, and O157. Here we present results of a blinded multilaboratory collaborative study involving 10 participants from nine laboratories using 55 unknown strains. From the total 495 analyses, two false-positive and three false-negative results were obtained, indicating the assay to be a rapid, high-throughput, and robust method for identifying clinically relevant STEC serogroups.

Publisher

International Association for Food Protection

Subject

Microbiology,Food Science

Link

http://meridian.allenpress.com/jfp/article-pdf/76/5/867/1685875/0362-028x_jfp-12-468.pdf

Reference10 articles.

1. The Non-O157 Shiga-Toxigenic (Verocytotoxigenic)Escherichia coli; Under-Rated Pathogens

2. Shiga toxin-producing Escherichia coli: An overview1

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