Occurrence of Scirpentriol and its Seven Acetylated Derivatives in Culture Extracts of Fusarium sambucinum NRRL 134951

Abstract

Ethyl acetate extracts of culture filtrates from Fusarium sambucinum NRRL 13495 contained scirpentriol, triacetoxyscirpenol, 4,15-diacetoxyscirpenol, 3,4-diacetoxyscirpenol, 3,15-diacetoxyscirpenol, 15-monoacetoxyscirpenol, 4-monoacetoxyscirpenol, and 3-monoacetoxyscirpenol which are the eight possible members of the scirpentriol family of trichothecene mycotoxins. These eight mycotoxins were identified by chemical derivatization, thin layer chromatography, gas chromatography, mass spectroscopy, nuclear magnetic resonance, and comparison to standards. The total concentration of scirpenol derivatives in the culture filtrate was 426 mg/liter of which 346 mg/L was 4,15-diacetoxyscirpenol. The occurrence of a complete family of mycotoxins in a single culture implies that outbreaks of toxicity associated with Fusarium may be multiple toxicoses, and that the screening of foodstuffs for trichothecenes should be broadened, particularly potato tubers which are subject to infection by F. sambucinum.