Millipore Filtration and Use of RV Medium for Isolation of Salmonella from Preenrichment Broths

Abstract

A filtration procedure for isolation of Salmonella from preen-richment broth is described. Five ml of broth are passed through a Millipore AP15 pre-filter above a .45 μm Millipore filter. The filter is transferred to 25 ml of Rappaport-Vassiliadis broth and incubated at 42°C. Of 76 naturally and 55 artificially contaminated meat and poultry samples, 102 were positive for Salmonella. Of these, 99 were isolated using the filtration technique, 93% of which were obtained following a 6-h incubation period. Isolations from tetrathionate brilliant green and selenite cystine broth were 93 and 84, respectively.