Resistance to Gentamicin and Vancomycin in Enterococcal Strains Isolated from Retail Broiler Chickens in Japan

Abstract

A total of 137 Enterococcus strains isolated from chicken meat were subjected to antimicrobial susceptibility tests. Strains with the vanC1 gene were isolated from seven of nine samples of chicken meat processed in Japan and from all chickens from China and Brazil between July 2001 and April 2002. The pulsed-field gel electrophoresis (PFGE) patterns of the isolates were distinguishable from each other, suggesting that VanC1-type vancomycin-resistant Enterococcus is preferentially colonized in broiler chickens in these countries. The incidence of high-level gentamicin resistant (HLGR) enterococci that harbored the aac(6′)-Ie-aph(2″)-Ia or aph(2′)-Id gene varied among the countries from which the chickens originated (Japan, 2 of 65; China, 11 of 43; Brazil, 6 of 29). Moreover, the PFGE patterns of the HLGR strains were distinguishable from each other, except for two strains obtained from chickens from Brazil. The results suggest that HLGR Enterococcus is highly prevalent in broiler chickens.