Evaluation of an Enrichment-Plating Procedure for the Recovery of Campylobacter jejuni from Turkey Eggs and Meat

Abstract

A selective enrichment-plating procedure was tested for the recovery and enumeration of Campylobacter jejuni from turkey eggs and meat. Enrichment was in brucella broth with ferrous sulfate, sodium metabisulfite, sodium pyruvate and five antimicrobial agents. Plating was on brucella agar supplemented with equine blood and antimicrobial agents. Incubation of tubes and plates was at 42°C in an atmosphere of 5% O2:10% CO2:85% N2. C. jejuni could be recovered from the enrichment broth when calculated initial cell numbers per ml of broth were as low as 0.3 to 3.3