Microbiological Quality of Chilled Beef Carcasses in Northern Ireland: A Baseline Survey

Abstract

To standardize the assessment of the hygienic quality of beef carcasses in Northern Ireland (NI) abattoirs, swabbing techniques were evaluated. Six materials, including two commercially produced swabs, were compared for their ability to recover spoilage and pathogenic bacteria and for their ease of use as carcass swabs. A sponge retailed for domestic use was selected on the basis of efficiency of recovery of microorganisms, ease of use, and cost. On sample carcasses, 1,000 cm2 of the brisket was swabbed, since this site is normally readily contaminated. For 9 months, 420 carcasses in seven of the nine European Union–approved abattoirs in NI were sampled while in the chiller (24 to 48 h after kill). Total viable count (TVC), yeasts and molds, and Enterobacteriaceae were enumerated after incubation at 22 (48 h) and 37°C (48 h), and the results were expressed as log CFU/cm2. The mean TVC results at 22 and 37°C were 2.80 ± 0.70 and 2.75 ± 0.64, respectively. Although 63% of samples had yeasts that grew at 22°C, only 35% were positive at 37°C. The respective mean yeast counts were 1.12 ± 0.59 and 0.46 ± 0.51. Enterobacteriaceae were present in 15% of samples at 22°C and 21% of samples at 37°C. The mean counts for positive samples were 0.41 ± 0.37 and 0.40 ± 0.30, respectively. Molds were found in less than 4% of samples. Given that the brisket is normally one of the most heavily contaminated parts of the carcass, these results suggest that good hygienic practices are in operation in NI abattoirs. The results also enabled the abattoirs with the cleanest carcasses to be identified, hence permitting best practices to be found.