Detection of Mold in Food by Enzyme-Linked Immunosorbent Assay-Reference-Cited by-同舟云学术

Detection of Mold in Food by Enzyme-Linked Immunosorbent Assay

Published:1986-10-01 Issue:10 Volume:49 Page:786-791
ISSN:0362-028X
Container-title:Journal of Food Protection
language:en
Short-container-title:

Author:

NOTERMANS S.¹,HEUVELMAN C. J.¹,VAN EGMOND H. P.¹,PAULSCH W. E.¹,BESLING J. R.¹

Affiliation:

1. National Institute of Public Health and Environmental Hygiene, P.O. Box 1, 3720 BA Bilthoven, The Netherlands and Food Inspection Service, Baan 7, 3011 CD Rotterdam, The Netherlands

Abstract

Evaluation of the enzyme-linked immunosorbent assay (ELISA) for detecting a mold-specific, heat-stable and water-soluble antigen demonstrated the potential of the method for detecting molds in food products. The mold antigen, as produced by Penicillium spp. and Aspergillus spp., was present in all food samples containing aflatoxin B1. The amount of mold antigen present in the test samples was related in each case to the aflatoxin B1 content. Experiments done with samples artificially inoculated with mycotoxin-producing molds revealed that mold contamination could be detected by ELISA at a very early stage. The minimum detectable amount of mold mycelium for three different species of Penicillium was 38 ng/g of sample.

Publisher

International Association for Food Protection

Subject

Microbiology,Food Science

Link

http://meridian.allenpress.com/jfp/article-pdf/49/10/786/1656937/0362-028x-49_10_786.pdf

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