Development and Model Testing of Antemortem Screening Methodology To Predict Required Drug Withholds in Heifers†

Author:

JONES SHUNA A.12,SALTER ROBERT S.3,GOLDSMITH TIM4,QUINTANA JULIO3,RAPNICKI PAUL5,SHUCK KAREN2,WELLS JIM E.1,SCHNEIDER MARILYN J.6,GRIFFIN DEE2

Affiliation:

1. 1U.S. Department of Agriculture, Agricultural Research Service, U.S. Meat Animal Research Center, Clay Center, Nebraska 68933-0166

2. 2Great Plains Veterinary Educational Center, University of Nebraska Lincoln, Clay Center, Nebraska 68933

3. 3Charm Sciences, Inc., 659 Andover Street, Lawrence, Massachusetts 01843

4. 4College of Veterinary Medicine, Center for Animal Health and Food Safety, University of Minnesota, Minneapolis, Minnesota 55455

5. 5Elanco Animal Health, 2500 Innovation Way, P.O. Box 708, Greenfield, Indiana 46140

6. 6U.S. Department of Agriculture, Eastern Regional Research Center, 600 East Mermaid Lane, Wyndmoor, Pennsylvania 19038, USA

Abstract

A simple, cow-side test for the presence of drug residues in live animal fluids would provide useful information for tissue drug residue avoidance programs. This work describes adaptation and evaluation of rapid screening tests to detect drug residues in serum and urine. Medicated heifers had urine, serum, and tissue biopsy samples taken while on drug treatment. Samples were tested by rapid methods and high-performance liquid chromatography (HPLC). The adapted microbial inhibition method, kidney inhibition swab test, was useful in detecting sulfadimethoxine in serum, and its response correlated with the prescribed withdrawal time for the drug, 5 to 6 days posttreatment. The lateral flow screening method for flunixin and beta-lactams, adapted for urine, was useful in predicting flunixin in liver detected by HPLC, 96 h posttreatment. The same adapted methods were not useful to detect ceftiofur in serum or urine due to a lack of sensitivity at the levels of interest. These antemortem screening test studies demonstrated that the method selected, and the sampling matrix chosen (urine or serum), will depend on the drug used and should be based on animal treatment history if available. The live animal tests demonstrated the potential for verification that an individual animal is free of drug residues before sale for human consumption.

Publisher

International Association for Food Protection

Subject

Microbiology,Food Science

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