Affiliation:
1. Institute of Agro-Food Technology
2. Institute of Agricultural Quality Standard and Testing Technology, Jilin Academy of Agricultural Sciences, Changchun 130033, People's Republic of China
3. Clinical Medical College, Changchun University of Chinese Medicine, Changchun 130117, People's Republic of China
Abstract
ABSTRACT
Microbial enzymes within adjunct cultures are important for cheese ripening. Here, survival and proteolytic function of adjunct cultures of Lactobacillus plantarum strains MU12 and S6-4 on Cheddar cheese ripening were studied. Cheeses were ripened at 4°C, and samples were collected for analysis after 1, 30, 60, and 90 days. Lactococci numbers decreased by 2 to 3 log versus control, except in a few samples exhibiting significantly elevated numbers. Lactobacilli mainly originated from adjunct cultures, with lactobacilli numbers in adjunct-treated cheese significantly exceeding control numbers after day 30. Postripening, no significant differences were observed in composition (fat, protein, and moisture) and texture among cheeses, although observed significant differences in small nitrogen-containing compound levels (water-soluble nitrogen, trichloroacetic acid–soluble nitrogen, and phosphotungstic acid–soluble nitrogen) reflected proteolytic differences during ripening. Hydrolyzed protein, free amino acids, and volatile levels were consistently higher in adjunct-treated versus control cheeses and affected flavor. Cheddar cheeses may serve to effectively deliver beneficial organisms possessing proteolytic function.
HIGHLIGHTS
Publisher
International Association for Food Protection
Subject
Microbiology,Food Science
Reference30 articles.
1. Albenzio,
M.,
Santillo
A.,
Caroprese
M.,
Ruggieri
D.,
Napolitano
F.,
and
SeviA.
2013.
Physicochemical properties of Scamorza ewe milk cheese manufactured with different probiotic cultures.
J. Dairy Sci.
96:
2781–2791.
2. Banks,
J. M.,
Yvon
M.,
Gripon
J. C.,
de la Fuente
M. A.,
Brechany
E. Y.,
Williams
A. G.,
and
MuirD. D.
2001.
Enhancement of amino acid catabolism in Cheddar cheese using α-ketoglutarate: amino acid degradation in relation to volatile compounds and aroma character.
Int. Dairy J.
11:
235–243.
3. Bergamini,
C. V.,
Hynes
E. R.,
Palma
S. B.,
Sabbag
N. G.,
and
ZalazarC. A.
2009.
Proteolytic activity of three probiotic strains in semi-hard cheese as single and mixed cultures: Lactobacillus acidophilus, Lactobacillus paracasei and Bifidobacterium lactis.
Int. Dairy J.
19:
467–475.
4. Bergamini,
C. V.,
Hynes
E. R.,
and
ZalazarC. A.
2006.
Influence of probiotic bacteria on the proteolysis profile of a semi-hard cheese.
Int. Dairy J.
16:
856–866.
5. Bezerra,
T. K.,
de Araujo
A. R.,
do Nascimento
E. S.,
de Matos Paz
J. E.,
Gadelha
C. A.,
Gadelha
T. S.,
Pacheco
M. T.,
do Egypto Queiroga
R. C. R.,
de Oliveira
M. E. G.,
and
MadrugaM. S.
2016.
Proteolysis in goat “coalho” cheese supplemented with probiotic lactic acid bacteria.
Food Chem.
196:
359–366.
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