Selecting a Miniaturized System for Identification of Enterobacteriaceae

Abstract

The most commonly used commercial diagnostic kits for identification of Enterobacteriaceae are API, Enteric-Tek, Enterotube II, Micro-ID, Minitek and Spectrum-10. The accuracy of identification by all systems does not vary significantly, and falls within the acceptable range. Therefore, a bacteriologist who is considering the use of these products should evaluate factors other than accuracy when making a choice. Twenty-three professional microbiologists who had previous experience with these systems listed advantages and disadvantages of each system, and evaluated the conventional procedure for identification. The comments were summarized and presented in tabular form. The current cost per isolate of each system and the cost of the identification manual, reagents and incidental costs were also determined. These data provide the potential user with comparative information on price, shelf-life, versatility, time required for inoculation, incubation and manipulation after incubation, possible difficulties in determining positive and negative reactions, and potential safety factors for laboratory personnel.