Gas-Liquid Chromatographic Differentiation Between Salmonella gallinarum and Salmonella pullorum

Author:

RICHTER E. R.1,BURNS M. C.1,BANWART G. J.1,RHEINS M. S.1

Affiliation:

1. Department of Microbiology, The Ohio State University, Columbus, Ohio 43210

Abstract

Salmonella gallinarom and Salmonella pullorom have been considered as one serovar, S. gallinarom-pullorom or S. gallinarom. This serovar possesses group D somatic antigens with no flagellar antigen. Reportedly S. gallinarom differs from S. pullorom in dulcitol fermentation. This reaction is positive, but delayed up to 5 d for S. gallinarom and negative for S. pullorom. Gas-liquid chromatography of organic acid by-products from a dulcitol medium was performed on 10 isolates of each biovar. Viable plate counts confirmed approximately the same number of organisms per ml of culture. Results of pH determinations supported gas-liquid chromatographic analysis of more acid formation in all S. gallinarom cultures as compared with the S. pullorom cultures after incubation for 24 h. A quantitative measurement of succinic acid resulted in confirmation of the differences in metabolic function of both biovars. The additional test procedure of gas-liquid chromatography of organic acid by-products aids the clinician or researcher in rapidly and accurately distinguishing these two similar biovars.

Publisher

International Association for Food Protection

Subject

Microbiology,Food Science

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