Affiliation:
1. 1Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun 130122, People's Republic of China
2. 2National Engineering Laboratory for Druggable Gene and Protein Screening, Northeast Normal University, Changchun 130117, People's Republic of China
Abstract
Cronobacter sakazakii is an emerging pathogen that causes severe and life-threatening conditions including meningitis, bacteremia, and necrotizing enterocolitis. An animal model study for extrapolation of C. sakazakii infection can provide a better understanding of pathogenesis. However, methods for real-time monitoring of the course of C. sakazakii infection in living animals have been lacking. We developed a bioluminescent C. sakazakii strain (ATCC 29544) that can be used for real-time monitoring of C. sakazakii infection in BALB/c mice. C. sakazakii ATCC 29544 mainly colonized brain, liver, spleen, kidney, and gastrointestinal tract, as indicated by bioluminescence imaging. This work provides a novel approach for studying the progression of C. sakazakii infection and evaluating therapeutics in a living mouse model.
Publisher
International Association for Food Protection
Subject
Microbiology,Food Science
Cited by
7 articles.
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