Usefulness of the Benzalkon-Crystal Violet-ATP Method for Predicting the Keeping Quality of Pasteurized Milk

Abstract

Using the benzalkon-crystal violet-ATP method (BC-ATP method), post-pasteurization contamination of pasteurized milk caused by gram-negative bacteria can be determined within 24 h. This study determined to what extent the keeping quality of pasteurized milk can be predicted by applying this BC-ATP method. Results obtained with the BC-ATP method for 100 samples of pasteurized milk were compared with those recorded in the shelf-life test (total bacterial count after 10 d of storage of samples at 7°C; standard: 106 bacteria per ml) and the Moseley test (bacterial count after 5 d of storage at 7°C; standard: 105 bacteria per ml). Using the shelf life test and the Moseley test, 14 and 8% of the results, respectively, did not correspond with those obtained with the BC-ATP method. From the results obtained, it was obvious that the greater the post-pasteurization contamination of the pasteurized milk, the lesser is the keeping quality. A quantitative estimation of the degree of post-pasteurization contamination can be obtained satisfactorily by applying the BC-ATP method to 1000-, 100-, 10- and 1-ml portions. The Moseley test takes too much time to achieve a good coordination between the factory laboratory and the pasteurization and filling sections of the dairy factory. By substituting the BC-ATP method applied to 100-ml portions of pasteurized milk for the Moseley test (5 d at 7°C; standard: 100,000 bacteria per ml) almost the same information is obtained within 24 h.