Gamma Radiation Resistance of Clostridium botulinum 62A and Bacillus subtilis Spores in Honey

Author:

HUHTANEN C. N.1

Affiliation:

1. United States Department of Agriculture, ARS, Eastern Regional Research Center, 600 E. Mermaid Lane, Wyndmoor, Pennsylvania 19118

Abstract

Irradiation D values for the natural bacterial flora of two samples of raw (bulk) honey were 7.50 and 1.91 kGy; for two samples of retail honey the D values were 5.66 and 3.49 kGy. Irradiation D values of Clostridium botulinum 62A spores inoculated into three honey samples and into water were respectively, 8.11, 9.38, 12.77, and 2.07 kGy. Similar D values for Bacillus subtilis spores were 3.42, 3.35, 4.00, and 1.43 kGy. The radiation resistance of C. botulinum and B. subtilis spores in honey and in sugar syrups was a function of water content.

Publisher

International Association for Food Protection

Subject

Microbiology,Food Science

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