Prevalence and Multidrug Resistance of Salmonella in Swine Production Chain in a Central Province, Thailand-Reference-Cited by-同舟云学术

Prevalence and Multidrug Resistance of Salmonella in Swine Production Chain in a Central Province, Thailand

Published:2021-08-19 Issue:12 Volume:84 Page:2174-2184
ISSN:0362-028X
Container-title:Journal of Food Protection
language:en
Short-container-title:

Author:

CHONSIN KAKNOKRAT¹^ORCID,CHANGKWANYEUN RUCHIRADA²,SIRIPHAP ACHIRAYA³,INTARAPUK APIRADEE⁴,PRAPASAWAT WATSAWAN⁴,CHANGKAEW KANJANA²,PULSRIKARN CHAIWAT⁵,ISODA NORIKAZU⁶,NAKAJIMA CHIE⁶⁷,SUZUKI YASUHIKO⁶⁷^ORCID,SUTHIENKUL ORASA²⁸^ORCID

Affiliation:

1. Faculty of Science and Technology, Suratthani Rajabhat University, Surat Thani 84100, Thailand

2. Faculty of Public Health, Thammasart University, Pathum Thani 12121, Thailand

3. Department of Microbiology and Parasitology, School of Medical Sciences, University of Phayao, Phayao 56000 Thailand

4. Department of Clinic, Faculty of Veterinary Medicine, Mahanakorn University of Technology, Bangkok 10530, Thailand

5. National Institute of Health, Department of Medical Science, Ministry of Public Health, Nonthaburi 11000, Thailand

6. International Collaboration Unit, Hokkaido University International Institute for Zoonosis Control, Sapporo, Hokkaido 060-0808, Japan

7. Division of Bioresources, Hokkaido University International Institute for Zoonosis Control, Sapporo, Hokkaido 001-0020, Japan

8. Faculty of Public Health, Mahidol University, Bangkok 10400, Thailand

Abstract

ABSTRACT Salmonella causes foodborne disease outbreaks worldwide and raises concerns about public health and economic losses. To determine prevalence, serovar, antimicrobial resistance patterns, and the presence of extended-spectrum β-lactamase (ESBL) genes in a cross-sectional study, 418 total samples from feces and carcasses (from three slaughterhouses) and pork and cutting boards (from four markets) were collected in a central Thailand province in 2017 and 2018. Of the 418 samples, 272 (65.1%) were positive for Salmonella. The prevalence of Salmonella-positive samples from markets (158 of 178; 88.8%) was significantly higher than that among samples from slaughterhouses (114 of 240; 47.5%) (P < 0.05). A total of 1,030 isolates were identified; 409 were assigned to 45 serovars, with Salmonella Rissen the most common (82 of 409; 20%). Two serovars, Salmonella Cannstatt and Salmonella Braubach, were identified for the first time in Thailand in market and slaughterhouse samples, respectively. Among 180 isolates representing 19 serovars, 133 (73.9%) exhibited multidrug resistance. Screening for ESBL production revealed that 41 (10.3%) of 399 isolates were ESBL positive. The prevalence of ESBL-producing Salmonella isolates was significantly higher among the market isolates (31 of 41; 75.6%) than among the slaughterhouse isolates in (10 of 41; 24.4%) (P < 0.05). In market samples, 24 (77.4%) of 31 isolates were recovered from pork and 7 (22.6%) were recovered from cutting boards. Nine ESBL-producing isolates carried single ESBL genes, either blaTEM (4 of 41 isolates; 9.8%) or blaCTX-M (5 of 41 isolates; 12.2%), whereas 11 (26.8%) carried both blaTEM and blaCTX-M. No ESBL-producing Salmonella isolate carried the blaSHV gene. These results suggest that pigs, their flesh, and cutting boards used for processing pork could be reservoirs for widespread ESBL-producing Salmonella isolates with multidrug resistance and outbreak potential across the food chain. HIGHLIGHTS

Publisher

International Association for Food Protection

Subject

Microbiology,Food Science

Link

https://meridian.allenpress.com/jfp/article-pdf/84/12/2174/2969770/i0362-028x-84-12-2174.pdf

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