Expression of the Low-density Lipoprotein Receptor (LDLR) Gene Family in CD133+/CD44+ Prostate Cancer Stem Cells-Reference-Cited by-同舟云学术

Expression of the Low-density Lipoprotein Receptor (LDLR) Gene Family in CD133+/CD44+ Prostate Cancer Stem Cells

Published:2023-01-31 Issue:1 Volume:7 Page:410-417
ISSN:2564-7288
Container-title:Journal of Basic and Clinical Health Sciences
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Author:

SONER Burak Cem¹^ORCID,AÇIKGÖZ Eda²^ORCID,DUZAGAC Fahriye³^ORCID,PARLAYAN Cuneyd⁴^ORCID

Affiliation:

1. IZMIR DEMOCRACY UNIVERSITY

2. VAN YÜZÜNCÜ YIL ÜNİVERSİTESİ, TIP FAKÜLTESİ, TEMEL TIP BİLİMLERİ BÖLÜMÜ, HİSTOLOJİ VE EMBRİYOLOJİ ANABİLİM DALI

3. M.D. Anderson Cancer Center, 3Department of Clinical Cancer Prevention, The University of Texas

4. BAHCESEHIR UNIVERSITY, SCHOOL OF MEDICINE, DEPARTMENT OF BASIC MEDICAL SCIENCES, DEPARTMENT OF BIOSTATISTICS AND INFORMATICS MEDICINE

Abstract

Aim: The low-density lipoprotein receptor gene (LDLR) family plays a fundamental role in many malignancies and may have a putative cancer-boosting function. In our study, we have attempted to comparatively investigate the differential gene expressions of LDLR family in normal prostate epithelial cell line (RWPE-1), prostate cancer cell line (DU145 cell line), prostate cancer stem cells (DU145 CSCs) and non-CSCs (DU145 non-CSCs, bulk population). Materials and Methods: Cancer stem cells in DU-145 prostate cancer cell line were isolated by flow cytometry according to CD133 and CD44 cell surface properties. Whole transcriptome sequencing data was comprehensively analyzed for each group. The protein-protein interaction network was determined using the STRING protein database. Results: Our data showed that the expression levels of LRP1, LRP3, LRP8 and LRP11 were increased in the DU145 CSCs relative to the normal prostate epithelial cell line. Conclusion: Overall, our data suggest that the LRP functions and/or the expression in prostate cancer may ultimately change the invasive phenotype of the CSCs.

Publisher

Dokuz Eyul Universitesi Saglik Bilimleri Enstitusu

Subject

General Medicine

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