Brine shrimp cytotoxic activity of methanolic extracts of Juniperus communis L. berries

Abstract

Methanolic extracts from Juniperus communis L. berries collected from five different localities in the Republic of Macedonia were evaluated for their cytotoxicity by Brine shrimp lethality assay. The obtained cytotoxic activity is descending as follows: Pelister (128 μg/ mL) > Jakupica (221 μg/mL) > Prilep (662 μg/mL) > Demir Hisar (863 μg/mL) > Makedonski Brod (969 μg/mL). Berries collected from mountain areas (Pelister and Jakupica) demonstrated prominent cytotoxic effects, while berries collected from localities near urban areas exhibited lower cytotoxicity. Variations in their bioactivity are probably due to their complex phytochemical composition, which may vary with different ecological and geographical conditions.