Luminescence measurements: reference materials for composition of sodium adenosine triphosphate aqueous solution

Abstract

The authors evaluated the possibility of using the luminometry method which allows checking the level of sanitation and hygiene in industries and is one of the most effective methods of «rapid microbiology». Such key stages of development of reference material for sodium adenosine triphosphate with the certified values as analysis of similar reference materials, selection of source material of the reference material, conducting experimental studies, and establishing the metrological characteristics of reference material with traceability to state primary measurement standard were provided in the paper. Considering that the selective optical density at the characteristic wavelength is directly proportional to the concentration of the absorbing component (sodium adenosine triphosphate), the stability of R M was studied by spectrophotometry. The stability of the RM material to assess the uncertainty of the measurement results was carried out by the isochronous method. The result of the study is the possibility of ensuring the traceability of measurement results based on luminescence to primary measurement standard by sodium adenosine triphosphate concentration. In addition to the above, the authors selected the optimal value of that concentration and established metrological properties for GSO 11606-2020 R M for the composition of sodium adenosine triphosphate aqueous solution (mass concentration: from 0,09 to 0,11 g/dm3; extended uncertainty: 0,015 g/dm3). Usage of this CRM in performing tests, calibrations, and verification of measuring instruments allows providing the unbiased assessment of the measuring capabilities of luminescent measuring instruments, including in terms of determining the error of the measurements carried out.