BIOCOMPATIBLE AND MATRIX PROPERTIES OF POLYLACTIDE SCAFFOLDS-Reference-Cited by-同舟云学术

BIOCOMPATIBLE AND MATRIX PROPERTIES OF POLYLACTIDE SCAFFOLDS

Published:2018-06-27 Issue:2 Volume:20 Page:82-90
ISSN:2412-6160
Container-title:Russian Journal of Transplantology and Artificial Organs
language:
Short-container-title:RJTAO

Author:

Sevastianov V. I.¹,Grigoriev A. M.¹,Basok Yu. B.¹,Kirsanova L. A.¹,Vasilets V. N.²,Malkova A. P.³,Dukhina G. A.³,Grigoriev T. E.⁴,Zagoskin Yu. D.⁵,Tokaev K. V.⁶,Tokaev T. K.⁷

Affiliation:

1. V.I. Shumakov National Medical Research Center of Transplantology and Artiﬁ cial Organs of the Ministry of Healthcare of the Russian Federation.

2. V.I. Shumakov National Medical Research Center of Transplantology and Artiﬁ cial Organs of the Ministry of Healthcare of the Russian Federation; V.L. Talrose Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences.

3. Institute of Biomedical Research and Technology.

4. National Research Center «Kurchatov Institute»; A.N. Nesmeyanov Institute of Organoelement Compounds of Russian Academy of Science.

5. National Research Center «Kurchatov Institute».

6. National Medical Research Center of Phthisiopulmonology and Infection Diseases of the Ministry of Healthcare of the Russian Federation.

7. Moscow City Research and Practical Center for Tuberculosis Treatment, Moscow Health Department.

Abstract

Aim.A study of biocompatible and matrix properties of polylactide scaffolds as a materials for medical implanted articles as well as scaffolds for cell and tissue engineering constructions.Materials and methods. Biocompatibility of polylactide scaffolds in the form of porous disks obtained by freeze drying method was estimated in vitro: by UV spectroscopy, pH measurements and cytotoxicity to NIH/3T3 mice ﬁ broblasts in static conditions. Biocompatibility of scaffolds in vivo was investigated by its implantation under mice skin. Matrix properties of polylactide scaffolds (cell adhesion and proliferation) were studied in dynamic conditions with mesenchymal stromal cells of human adipose tissue (MSC ADh) in perfusion bioreactor.Results.As a result of in vitro investigations it was shown that polylactide scaffolds obtained by freeze drying are satisﬁ ed to demands presenting biocompatible medical articles with respect to pH measurements, extraction tests and cyto toxicity to mice ﬁ broblasts NIH/3T3. Cultivation of MSC ADh in perfusion bioreactor in hepatogenic media is accompanying by good adhesion and proliferation both on the surface and in the bulk of porous disks. However implantation of polylactide scaffolds under mice skin is accompanying by resorption and leads to the inﬂ ammation reaction of adjacent tissues.Conclusions.Positive results obtained only by in vitro testing of biocompatibility and matrix properties are not enough to recommend the material to be used as a scaffolds for cell and tissue engineering constructions. The preliminary study of biomechanical characteristics of the implant is recommended.

Publisher

V.I. Shimakov Federal Research Center of Transplantology and Artificial Organs

Subject

Transplantation,Immunology and Allergy

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