Influence of rat bone marrow mesenchymal stem cells to rat Langerhans islets viability during co-cultivation with microst ructured collagen‑containing hydrogel

Abstract

Aim. To research the rat bone marrow mesenchymal stem cells (MSC BM) effect to the viability of isolated rat OL during cultivation with microstructured collagen-containing hydrogel (BMCH).Materials and methods. Rat islets and MSC BM were isolated according to  classical technique with some modifications. The experiments on the co- cultivation were carried out under standard conditions. Islet’s viability was  determined by immunofluorescence staining.Results. TheOL cultivation with BMCH and MSCBM resulted in destructive changes of isletson the third day, and complete destruction of OL was observed  on the seventh day. At the same time, OL cultured with BMCH remained viable for 14 days of incubation.Conclusion. Cultivation of OL with BMCH and MSC BM did not contribute to the  long-term survival of the islets. Thus, there was no positive effect of MSC BM on the viability of pancreatic islets.