ACUTE ORAL TOXICITY PARAMETERS OF THE COMBINED ANTIPARASITIC DRUG IN THE FORM OF SPRAY

Abstract

The purpose of the research was to study acute oral toxicity parameters of the combined veterinary Fipronil-, Pyriproxyfen- and Moxidectin-based drug in the form of spray. The studies were performed in the vivarium on 50 outbred white male mice weighing 17–20 g and on 30 outbred white male rats weighing 185–205 g. The drug for the mice was diluted in PEG-400 in the ratio 1:3 and administered by an intragastric tube; we tested doses of 1025 mg/kg, 3075 mg/kg, 5125 mg/kg and 7175 mg/kg. The drug was administered to the rats without dilution; we tested doses of 1640 mg/kg, 3690 mg/kg, 5740 mg/kg and 7380 mg/kg. The animals’ physiological state, intoxication symptoms and possible death were monitored within 14 days. As a result of the studies, toxic effects of the drug were determined and calculated using the Miller-Tainter method. The LD50 of the drug was 4100.0±560.3 mg/kg for mice, and 4458±690.5 mg/kg for rats, on the basis of which it was found that the drug was classified as moderately hazardous substance (hazard category 3 substance) according to GOST 12.1.007-76.