Understanding physiological and molecular adaptations of three diverse halophytic grasses under saline and sodic stresses

Abstract

Physiological and biochemical analyses of three halophytes were conducted to explore their tolerance and phytoremediation potential. The halophytes Urochondra setulosa, Sporobolus marginatus and Leptochloa fusca were categorised based on gas exchange attributes, antioxidant system, biomass production under soil salinity (ECe 30-50 dS/m) and sodicity (pH 9.5-10). L. fusca and S. marginatus produced slightly higher biomass under the sodic condition of pH 9.5, while in U. setulosa, it increased under salinity stress. Under sodic conditions, L. fusca showed less reduction in potassium content and maintained a higher K+/Na+ ratio in their leaf tissues. A maximum decrease in net photosynthesis was observed in L. fusca (28.55%) at ECe ~ 50 dS m-1 while minimum in S. marginatus (13.73%) at pH ~ 10.0. Comparatively, U. setulosa showed higher stomatal conductance and transpiration rate than L. fusca and S. marginatus. At the highest pH and salinity, the antioxidant activities of enzymes APX, SOD, GR and POX increased in all three halophytes. Quantitative expression of MnSOD, NHX1 and FuSOS1 genes in all three halophytes increased with salt stresses. Based on these indicators, these halophytes were categorised as salt-tolerant or alkaline-stress-tolerant.