Author:
Juache-Villagrana Alan Esteban,Ponce-Garcia Gustavo,Gonzalez-Escandon Mizael De J.,Vazquez-Dominguez Isaac F.,Castro-Bautista Andre G.,Lopez-Monroy Beatriz,Rodriguez-Sanchez Iram P.,Ojeda Maria G. Alvarez,Flores Adriana E.
Abstract
ABSTRACT
Rhipicephalus microplus is the most important tick in veterinary medicine, given its repercussions on animal production. The principal strategy to avoid adverse effects associated with R. microplus is the chemical control of tick populations through organosynthetic acaricides. Therefore, monitoring susceptibility to acaricides is paramount in any control program. This study aimed to analyze the resistance status of 2 populations of R. microplus from northeastern Mexico to the organochlorine (OC) lindane, organophosphates (OP) coumaphos, chlorfenvinphos, diazinon, and chlorpyrifos, and the synthetic pyrethroids (SPs) flumethrin, deltamethrin, and cypermethrin. Discriminating doses (DD) of each acaricide were used in the larval packet bioassay (LPT). Additionally, the presence of the knockdown resistance (kdr) mutation T2134A associated with pyrethroid resistance was evaluated using allele-specific polymerase chain reaction (PCR). The populations of R. microplus showed a high frequency of resistance to SP, with mortality rates of less than 5%; they also showed resistance to the OPs (diazinon and chlorpyrifos) with mortality rates ranging from 1.29% to 34.62%; meanwhile, they were susceptible to coumaphos and chlorfenvinphos. Mortality rates higher than 66% were observed for lindane, indicating susceptibility. The mutant allele of the kdr mutation T2134A was detected in 75% and 100% of the pools analyzed. The populations studied presented a highly resistant profile to pyrethroids, with the presence of the kdr mutant allele A2134. The susceptibility to the organophosphates such as coumaphos and chlorfenvinphos of R. microplus from northeastern Mexico should be noted.
Publisher
American Mosquito Control Association
Subject
Insect Science,Public Health, Environmental and Occupational Health,General Medicine,Ecology, Evolution, Behavior and Systematics
Reference58 articles.
1. Abbott
WS.
1925.
A method of computing the effectiveness of an insecticide.
J Econ Entomol8:
265–
267.
2. Aguirre
EJ,
Santamaría
VM.
1986.
Purificación y caracterización toxicológica de garrapatas B. microplus resistentes a ixodicidas organofosforados y organoclorados.
Memorias de IV Reunión Anual de la Asociación Mexicana de Parasitología Veterimaria.
A.C. Cd. Victoria Tamps,
México.
3. Alonso-Díaz
MA,
Fernández-Salas
A.
2022.
Ripicephalus microplus: biología, control y resistencia. Manual técnico.
Tlapacoyan, Veracruz, Mexico:
CEIEGT.
40p.
4. Betancur Hurtado
J,
Giraldo-Ríos
C.
2019.
Economic and health impact of the ticks in production animals.
In:AbubakarMand
PereraPK,eds.Ticks and tick-borne pathogens.
Rijeka, Croatia:
InTech Publishers.
p1–
19.
5. Castro-Janer
E,
Klafke
GM,
Capurro
ML,
Schumaker
TT.
2015.
Cross-resistance between fipronil and lindane in Rhipicephalus (Boophilus) microplus.
Vet Parasitol210:
77–
83.