Affiliation:
1. KAFKAS ÜNİVERSİTESİ, SAĞLIK BİLİMLERİ FAKÜLTESİ
Abstract
The aim of this study was to evaluate the changes induced in testicular tissue by administration of dandelion (Taraxacum officinale) extract and its effects on the release of enzymatic antioxidants SOD-2 by immunohistochemical methods in rats with experimental diabetes. Degenerative changes in the seminiferous tubule structure were observed in the testicular sections of the diabetes group. Separations between the basement membrane and the seminiferous epithelium, disruptions in the structure and localization of spermatogenic cells toward the lumen of the seminiferous tubules, thickening of the basement membrane and tunica albuginea, changes in the shape of Sertoli cells, and shrinkage of the nucleus of Leydig cells were observed. While no immunopositivity was observed in spermatogonium and primary spermatocytes in the diabetes group, weak SOD-2 immunoreactivity was observed in other cells of the spermatogenic series and moderate SOD-2 immunoreactivity was observed in the diabetes+dandelion group. In conclu-sion, it was found that histopathological changes occurred in the testicular tissue and SOD-2 immunoreactivity decreased in diabetes, a chronic metabolic disease. It was concluded that dandelion administration may have beneficial effects on decreased SOD-2 immunoreactivity.
Reference30 articles.
1. Al-Salmi AF, Hamza RZ. Efficacy of vanadyl sulfate and selenium tetrachloride as anti-diabetic agents against hyperglycemia and oxidative stress induced by diabetes mellitus in male rats. Curr Is-sues Mol Biol 2022; 44 (1): 94-104.
2. Bener A, Al-Ansari AA, Zirie M, Al-Hamaq AO. Is male fertility associated with type 2 diabetes melli-tus? Int Urol Nephrol 2009; 41(4): 777-84.
3. Choi UK, Lee OH, Yim JH, Cho CW, Rhee YK, Lim SI, Kim YC. Hypolipidemic and antioxidant effects of dandelion (taraxacum officinale) root and leaf on cholesterol-fed rabbits. Int J Mol Sci 2010; 11(1): 67-78.
4. Dongmei C. Atlas of Histology with Functional and Clinical Correlations. First Edition. Philadelphia: Wolters Kluwer Health/Lippincott Williams & Wil-kins, 2011; p.344-68.
5. Donmez YB, Kizilay G, Topcu-Tarladacalisir Y. MAPK immunoreactivity in streptozotocin-induced diabetic rat testis. Acta Cir Bras 2014; 29(10): 644-50.