Yem Kısıtlamasının Rat Testisinde PTGS2 ve NRF2 Genlerinin Ekspresyon Seviyelerine Etkileri-Reference-Cited by-同舟云学术

Yem Kısıtlamasının Rat Testisinde PTGS2 ve NRF2 Genlerinin Ekspresyon Seviyelerine Etkileri

Published:2022-08-01 Issue: Volume: Page:
ISSN:1304-7280
Container-title:Erciyes Üniversitesi Veteriner Fakültesi Dergisi
language:tr
Short-container-title:Erciyes Üniv Vet Fak Derg

Author:

ÖZKAN Hüseyin¹,DALKIRAN Sevda²,KARAASLAN İrem³,KAYA Ufuk¹,ALAKUŞ İbrahim¹,ÇAMDEVİREN Baran⁴,YAKAN Akın¹

Affiliation:

1. HATAY MUSTAFA KEMAL ÜNİVERSİTESİ, VETERİNER FAKÜLTESİ, VETERİNER PR.

2. HATAY MUSTAFA KEMAL ÜNİVERSİTESİ, SAĞLIK BİLİMLERİ ENSTİTÜSÜ, MOLEKÜLER BİYOKİMYA VE GENETİK (DR)

3. HATAY MUSTAFA KEMAL ÜNİVERSİTESİ, TEKNOLOJİ VE ARAŞTIRMA-GELİŞTİRME UYGULAMA VE ARAŞTIRMA MERKEZİ

4. HATAY MUSTAFA KEMAL ÜNİVERSİTESİ, SAĞLIK BİLİMLERİ ENSTİTÜSÜ, MOLEKÜLER BİYOKİMYA VE GENETİK ANABİLİM DALI

Abstract

This study aimed to investigate the effects of food restriction on oxidative status at the molecular levels of the testis in rats. A total of 16 male Wistar rats were allocated to two groups (n=8) and fed for four weeks. The first group was control (Cont) and consumed food and water ad libitum. The second one, food-restricted group (FR) was presented half of the regular consumption. But the water was presented ad libitum. The feeding period was maintained for four weeks. At the end of the feeding period, rats were euthanized by cardiac blood sampling under anesthesia. Malondialdehyde (MDA) levels in testis tissue were determined and calculated as nmol/mg protein. In addition, Prosta-glandin-Endoperoxide Synthase 2 (PTGS2) and Nuclear factor erythroid 2–related factor 2 (NRF2) genes expression levels were determined in testis tissue. While MDA and gene expression levels were found similar in groups, a positive correlation was found between PTGS2 and NRF2 genes (r=0.629; P

Publisher

Erciyes Universitesi

Subject

General Earth and Planetary Sciences,General Engineering,General Environmental Science

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