Molecular and morphological characterisation of Rotylenchus vitis n. sp. (Nematoda: Hoplolaimidae) infectinggrapevine in southern Spain

Author:

Cantalapiedra-Navarrete Carolina1,Liébanas Gracia2,Archidona-Yuste Antonio1,Palomares-Rius Juan E.1,Castillo Pablo1

Affiliation:

1. Institute for Sustainable Agriculture (IAS), Spanish National Research Council (CSIC), Alameda del Obispo s/n, Apdo.4084, 14080 Córdoba, Spain

2. Department of Animal Biology, Vegetal Biology and Ecology, University of Jaén, Campus ‘Las Lagunillas’ s/n, EdificioB3, 23071 Jaén, Spain

Abstract

Rotylenchus vitis n. sp., a new amphimictic species infesting soil and roots of commercial vineyards in southern Spain, is described. Rotylenchus vitis n. sp. is characterised by a truncate lip region with 7-9 annuli and continuous with the body contour, lateral fields areolated at pharyngeal region only, body without longitudinal striations, stylet length of 36-48 μm, vulva position at 47-57%, tail rounded to hemispherical with 13-21 annuli. Morphologically, this species is related to R. cazorlaensis, R. capitatus, R. elegans, R. fabalus, R. iranicus, R. labiodiscus, R. montanus and R. troncapitatus. The results of the phylogenetic analysis based on the sequences of the D2-D3 expansion regions of the 28S and ITS1-rRNA genes confirmed the species differentiation and the close molecular relationship between R. eximius and R. montanus, respectively.

Publisher

Brill

Subject

Agronomy and Crop Science,Ecology, Evolution, Behavior and Systematics

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