Characterization and expression analysis of β-catenin from Exopalaemon carinicauda (Holthuis, 1950) (Caridea, Palaemonidae) in embryonic development and immune response to bacteria-Reference-Cited by-同舟云学术

Characterization and expression analysis of β-catenin from Exopalaemon carinicauda (Holthuis, 1950) (Caridea, Palaemonidae) in embryonic development and immune response to bacteria

Published:2023-01-31 Issue:1 Volume:96 Page:19-33
ISSN:0011-216X
Container-title:Crustaceana
language:
Short-container-title:Crustac.

Author:

Huang Yi Chen¹²³,Xu Wei Kang¹²³,Zhu Hong Geng¹²³,Chen Jian Hua¹²³^ORCID

Affiliation:

1. Jiangsu Key Laboratory of Marine Biotechnology/School of Food Science and Engineering, Jiangsu Ocean University, Lianyungang 222005, P.R. China

2. Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Jiangsu Ocean University, Lianyungang, 222005, P.R. China

3. Jiangsu Key Laboratory of Marine Bioresources and Environment, Jiangsu Ocean University, Lianyungang 222005, P.R. China

Abstract

Abstract β-catenin is an important signal transduction protein in the canonical Wnt/β-catenin pathway, which is widely involved in the development of animals. However, the role of β-catenin in crustaceans is still unclear. In this study, the cDNA of β-catenin from Exopalaemon carinicauda (Holthuis, 1950) was identified and characterized. The open reading frame (ORF) is 2424 bp, which encodes 807 amino acids containing an N-terminal region of a GSK-β consensus phosphorylation site and a central region of 11 armadillo (ARM) repeats. The tissue distribution showed that β-catenin was highly expressed in ovary, testis, hepatopancreas, heart, and gills. During embryonic development, the transcript of β-catenin was relatively high in the zygote, and the thirty-two-cell stage, blastula stage, gastrula stage, and nauplius stage. The transcripts of β-catenin in haemocytes, gills, and hepatopancreas were significantly up-regulated after infection with Vibrio anguillarum Bergeman, 1909. These results indicate that β-catenin participates in the regulation of embryonic development and in the immune response in E. carinicauda.

Publisher

Brill

Subject

Animal Science and Zoology,Aquatic Science

Link

https://brill.com/downloadpdf/journals/cr/96/1/article-p19_3.xml

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