A TaqMan real-time PCR assay for detection of Meloidogyne hapla in root galls and in soil-Reference-Cited by-同舟云学术

A TaqMan real-time PCR assay for detection of Meloidogyne hapla in root galls and in soil

Published:2016 Issue:2 Volume:18 Page:147-154
ISSN:1388-5545
Container-title:Nematology
language:
Short-container-title:Nematol

Author:

Sapkota Rumakanta¹,Skantar Andrea M.²,Nicolaisen Mogens¹

Affiliation:

1. Department of Agroecology, Faculty of Science and Technology, Aarhus University, Forsøgsvej 1, 4200 Slagelse, Denmark

2. Nematology Laboratory, United States Department of Agriculture, Agricultural Research Service, Beltsville, MD 20705, USA

Abstract

Early detection and quantification of Meloidogyne hapla in soil is essential for effective disease management. The purpose of this study was to develop a real-time PCR assay for detection of M. hapla in soil. Primers and a TaqMan probe were designed for M. hapla detection. The assay detected M. hapla and showed no significant amplification of DNA from non-target nematodes. The assay was able to detect M. hapla in a background of plant and soil DNA. A dilution series of M. hapla eggs in soil showed a high correlation (, ) with Ct values. The assay could predict root-knot development in carrots by testing soils before planting. The assay could be useful for management decisions in carrot cultivation.

Publisher

Brill

Subject

Agronomy and Crop Science,Ecology, Evolution, Behavior and Systematics

Link

https://data.brill.com/files/journals/15685411_018_02_s003_text.pdf

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