Morphological and molecular characterisation of Longidorus pinus sp. n. (Nematoda: Longidoridae) from China and a key to known species of Longidorus in China

Author:

Xu Yumei1,Ye Weimin2,Wang Jianming1,Zhao Zengqi13

Affiliation:

1. 1Laboratory of Nematology, Department of Plant Pathology, Agronomy College, Shanxi Agricultural University, Taigu 030801, P.R. China

2. 2Nematode Assay Section, Agronomic Division, North Carolina Department of Agriculture and Consumer Services, Raleigh, NC 27601, USA

3. 3Landcare Research, Private Bag 92170, Auckland Mail Centre, Auckland 1142, New Zealand

Abstract

Summary Longidorus pinus sp. n. from China is characterised by females 3.3-4.9 mm long; lip region 8-12 μm diam., distinctly offset by a constriction; amphidial fovea pocket-shaped without basal lobes; a short odontostyle (67-81 μm); anteriorly located guide ring (30-38 μm); and short and conoid tail (31-40 μm long, c = 84-138, c′ = 1.2-1.9), with one or two pairs of caudal pores on each side. Males were not found. Three developmental juvenile stages were identified, the first-stage juvenile with conoid tail (c′ = 2.4-2.9). The polytomous key codes for the new species are: A2(3), B1, C23, D4, E1, F2, G23, H23, I1, J2, K6. Molecular phylogenetic analyses based on SSU, D2-D3 of LSU, ITS1 and ITS2 rDNA sequences indicate that L. pinus sp. n. is closely related to L. hangzhouensis, L. asiaticus, and L. camelliae, all of which apparently share a common Asiatic geographic origin. In addition, a key for identification of Longidorus found in China, based on morphological characters of both female and first-stage juvenile, is provided.

Publisher

Brill

Subject

Agronomy and Crop Science,Ecology, Evolution, Behavior and Systematics

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