Comparison of the penetration, development and reproduction of Meloidogyne javanica and M. graminicola on partially resistant Oryza sativa cultivars from East Africa

Abstract

Summary The host response to Meloidogyne javanica infection of selected Oryza sativa and NERICA (New Rice for Africa) genotypes that are commonly grown in Tanzania and East Africa was examined. The O. sativa genotypes ‘Komboka’ and ‘Supa’ appeared to be partially resistant. A further experiment showed that both genotypes are also partially resistant to M. graminicola infection. The host response of ‘Komboka’ and ‘Supa’ to M. javanica and M. graminicola was further examined and compared in indoor growth chamber conditions. The genotypes ‘TOG5674’, ‘TOG5675’, ‘CG11’ and ‘CG14’ (both O. glaberrima) were included as the resistant reference genotypes for M. graminicola and the genotype ‘UPLRi-5’ (O. sativa) was included as the susceptible reference genotype for both species of root-knot nematodes. Meloidogyne graminicola was more aggressive on ‘Komboka’ and ‘Supa’ than M. javanica. Significantly less root galling was observed on ‘Komboka’ and ‘Supa’ than on ‘UPLRi-5’. In ‘Komboka’ and ‘Supa’ significantly fewer second-stage juveniles (J2) were able to penetrate the roots, to develop into adult females and to reproduce compared with ‘UPLRi-5’. Differential emigration of J2 from the roots of ‘Komboka’ and ‘Supa’ compared with ‘UPLRi-5’ contributed to the observed partial resistance in these genotypes to M. graminicola and M. javanica. Nematodes that successfully penetrated and developed in ‘Komboka’ and ‘Supa’ showed aberrant phenotypes. ‘Supa’ and ‘Komboka’ may be recommended for use by farmers in M. javanica and M. graminicola-infested fields.