Antagonistic activity of essential oils and their main constituents extracted from Ajania fruticulosa and A. potaninii against Ditylenchus destructor

Abstract

Summary The antagonistic activities of essential oils of Ajania potaninii and A. fruticulosa against Ditylenchus destructor were determined by the liquid soak method in 96-well microplates. The main constituents of A. potaninii essential oil were 1,8-cineole (22.19%), (+)-camphor (12.84%), (−)-verbenol (13.84%) and borneol (12.67%). The results showed that the essential oils of A. fruticulosa (EC50 = 0.17 mg ml−1) and A. potaninii (EC50 = 0.27 mg ml−1) have antagonistic activity against D. destructor. The results also showed that 1,8-cineole (EC50 = 0.49 mg ml−1), myrtenol (EC50 = 0.40 mg ml−1) (A. fruticulosa) and (−)-verbenol (EC50 = 1.09 mg ml−1) (A. potaninii) have antagonistic activity. 1,8-Cineole is a key element affecting the antagonistic activity that was found in both essential oils. Several main constituents found in the essential oils had antagonistic effects against D. destructor. These results provide the basis for future development and utilisation of a substance to control D. destructor.