Affiliation:
1. Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
2. Departamento de Biología Animal, Biología Vegetal y Ecología, Universidad de Jaén, Campus Las Lagunillas, s/n, 23071, Jaén, Spain
Abstract
Summary
A new species of Ektaphelenchinae was recovered from the bark of a dead date palm (Phoenix dactylifera) tree, and from a soil sample collected from the rhizosphere of gramineous grasses in Iraq and Iran, respectively. It was assigned to the genus Ektaphelenchus mainly by the lack of a functional anus and rectum, and having a conical posterior body end (tail) in female, not ending to a filiform tip, and males lacking a bursa. The recovered populations were described based on morphological and molecular data. Ektaphelenchus phoenicis n. sp. is characterised by 346-441 μm long females, having three lines in lateral field, a 12.3-15.8 μm long stylet with wide lumen lacking basal knobs or swellings, high lip region separated from the rest of body by a depression, metacorpus elliptical with refractive valve plates almost in the middle to slightly posterior, excretory pore posterior to metacorpus, postvulval uterine sac (PUS) 9.3-14.8 μm long, usually containing sperm, posterior body end (tail) with ventrally bent distal end, males common in population with seven precloacal + cloacal + caudal papillae and slightly elevated cloacal lips under SEM, appearing as a differentiated flap-like structure under LM. The new species was morphologically compared with typologically similar species of Ektaphelenchus with a similar body size, namely: E. golestanicus, E. oleae and E. taiwanensis; a similar female posterior body end, namely: E. cupressi, E. kanzakii, E. prolobos and E. riograndensis; and E. goffarti having a similar postcloacal structure. The phylogenetic relationships of the new species with other relevant genera and species were reconstructed using near full length sequences of small and D2-D3 expansion segments of large subunit (SSU and LSU D2-D3) rDNA. In both inferred phylogenies, the newly generated sequences of Ektaphelenchus phoenicis n. sp. occupied a placement inside a maximally supported clade including sequences of three genera Ektaphelenchus, Ektaphelenchoides and Devibursaphelenchus.
Subject
Agronomy and Crop Science,Ecology, Evolution, Behavior and Systematics
Reference42 articles.
1. A low-cost technique to manufacture a container to process meiofauna for scanning electron microscopy;Abolafia, J.
2. Metodología para la realización de análisis morfológicos y morfométricos en nematodos de muestras de suelos de cuevas y otros habitats;Abolafia, J.
3. Description of Ektaphelenchoides caspiensis n. sp. (Nematoda: Ektaphelenchinae) from Iran;Aliramaji, F.
4. Morphological and molecular characterisation of Ektaphelenchoides tonekabonensis n. sp. (Rhabditida: Ektaphelenchinae) from northern Iran;Aliramaji, F.
5. Description of Ektaphelenchus berbericus n. sp. (Rhabditida: Ektaphelenchinae) from eastern Iran;Alvani, S.