A rapid method for the identification of the soybean cyst nematode Heterodera glycines using duplex PCR-Reference-Cited by-同舟云学术

A rapid method for the identification of the soybean cyst nematode Heterodera glycines using duplex PCR

Published:2001 Issue:4 Volume:3 Page:365-371
ISSN:1388-5545
Container-title:Nematology
language:
Short-container-title:Nematol

Author:

Subbotin Sergei¹,Moens Maurice²,Peng Deliang³

Affiliation:

1. 1Institute of Parasitology of Russian Academy of Sciences, Leninskii prospect 33, Moscow, 117071, Russia

2. 2Agricultural Research Centre, Crop Protection Department, Burg. Van Gansberghelaan 96, 9820 Merelbeke, Belgium

3. 3Institute of Plant Protection of Chinese Academy of Agricultural Sciences, Beijing 100094, China

Abstract

AbstractA method for rapid identification of juveniles and cysts of the soybean cyst nematode based on PCR with species specific primers is described. The PCR assay was tested on 53 populations originating from China, Russia, USA and Brazil. A single cyst or second stage juvenile of Heterodera glycines alone or in a mixture with other soil inhabiting nematodes was detectable.

Publisher

Brill

Subject

Agronomy and Crop Science,Ecology, Evolution, Behavior and Systematics

Link

https://brill.com/downloadpdf/journals/nemy/3/4/article-p365_8.xml

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