Author:
Kang Jae Soon,Choi Kwang Sik,Shin Sang Chul,Moon Il Sung,Lee Sang Gil,Lee Si Hyeock
Abstract
Abstract
Pine wood wilt disease caused by the pine wood nematode, Bursaphelenchus
xylophilus , has been a serious problem in the southern regions of Korea.
Efficient diagnosis of B. xylophilus from infected pine wood specimens is
critical for the management of this pest. Traditional microscopic
examination often results in an erroneous identification because a closely
related non-pathogenic species, B. mucronatus, has a great degree of
morphological similarity to B. xylophilus. In an attempt to search for
reliable molecular markers for the discrimination of these species, we have
cloned the 5S rRNA genomic DNA fragments containing both coding and
intergenic spacer (IGS) regions from B. xylophilus and B. mucronatus through
a homology-probing PCR strategy. Sequence analyses revealed that coding
sequences of the 5S rRNA gene from the two species are almost identical
(98.3% homology) but that the IGS sequences differ substantially between the
species. Based on the IGS sequence differences (69.7% homology), we designed
species-specific primer sets and developed a PCR-based diagnosis protocol
for the identification and discrimination of the two nematode species on a
molecular basis.
Subject
Agronomy and Crop Science,Ecology, Evolution, Behavior and Systematics
Cited by
50 articles.
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