Author:
Matsunaga Koji,Togashi Katsumi
Abstract
Abstract
Two species-specific PCR primer pairs were developed for identifying the two
nematode species, Bursaphelenchus xylophilus and B. mucronatus. The primer
pairs were developed from the sequence of ribosomal DNA (rDNA) repeats to
produce DNA fragments of different lengths by PCR amplification. The DNA
fragments for B. mucronatus and B. xylophilus were 210 bp and 557 bp,
respectively. When mixed, neither primer pair inhibited the PCR
amplification of the other. Five isolates of B. xylophilus and four isolates
of B. mucronatus showed different band profiles of PCR products between the
two species, but identical profiles among isolates of the same species.
Subject
Agronomy and Crop Science,Ecology, Evolution, Behavior and Systematics