Zebrafish (Danio rerio) behavioural response to an odorant mixture containing attracting and repelling odorants

Abstract

Abstract Odorants guide essential activities such as foraging and fleeing predators. Studies usually examine behavioural and physiological effects of individual odorants, while animals in the environment are exposed to multiple stimuli simultaneously. In this study, we exposed zebrafish to a mixture of attraction-evoking and aversion-evoking amino acids, and behavioural responses were observed. Attraction to l-alanine and avoidance to l-cysteine were observed, and so these amino acids were used to make the mixture (zebrafish also avoided l-serine, but this was weaker than with l-cysteine exposures). When exposed to the mixture, fish responded with avoidance, which suggests that aversion-evoking stimuli outweigh attraction-evoking stimuli. Attraction towards the mixture was seen only when the concentration of l-cysteine was decreased from 0.1 to 0.001 mM. Olfactory ablation surgery confirmed that the behaviours were olfactory-mediated. Overall, this study demonstrated that odorant stimuli that repel outweigh stimuli that attract until their concentration decreases by as much as 100-fold.