Exploring behavioral and hormonal flexibility across light environments in guppies from low-predation populations

Abstract

Phenotypic flexibility is essential for organisms to respond to changing environments. Guppies experience light environments that alter their visibility to conspecifics and predators. We used guppies from populations with low rates of predation by fish, but which may be subject to avian predators, to test the hypotheses that conspicuous behaviors and the androgens that mediate them are reduced under high light, and that cortisol levels are increased under high light because the perceived risk is stressful. We found reduced courtship, potentially driven by the reduced female response to courtship, under high light. Aggression and testosterone levels were higher in the absence of females. We found elevated androgen and decreased cortisol levels following social interactions, but no relationship between hormones and behavior, and no influence of light level on hormones. We forward explanations for these results and advocate understanding the flexible response to light environments in a range of guppy populations.