Effectiveness of Lugol’s iodine solution for long-term preservation of zooplankton samples for molecular analysis
Author:
Affiliation:
1. National Institute of Polar Research
2. Department of Ocean Sciences, Tokyo University of Marine Science and Technology
3. Department of Environmental Engineering for Symbiosis, Graduate School of Science and Engineering, Soka University
Publisher
The Plankton Society of Japan/The Japanese Association of Benthology
Subject
Ecology,Aquatic Science,Ecology, Evolution, Behavior and Systematics,Oceanography
Link
https://www.jstage.jst.go.jp/article/pbr/17/4/17_P170407/_pdf
Reference37 articles.
1. Andersen P, Throndsen J (2003) Estimating cell numbers. In: Manual on Harmful Marine Microalgae (eds Hallegraeff GM, Anderson DM, Cembella AD). UNESCO, Paris, p. 99–129.
2. Auinger B, Pfandl K, Boenigk J (2008) Improved methodology for identification of protists and microalgae from plankton samples preserved in Lugol’s iodine solution: combining microscopic analysis with single-cell PCR. Appl Environ Microbiol 74(8): 2505– 2510.
3. Blom MPK (2021) Opportunities and challenges for high-quality biodiversity tissue archives in the age of long-read sequencing. Mol Ecol 30(23): 5935–5948.
4. Bucklin A (2000) Methods for population genetic analysis of zooplankton. In: ICES zooplankton methodology manual (eds Harris R, Wiebe P, Lenz J, Skjoldal HR, Huntley M). Academic Press, London, p. 533–570.
5. Churro C, Valério E, Pereira P, Vasconcelos V (2015) Applicability of the real-time PCR assay in the amplification of cyanobacterial DNA from preserved samples. Limnetica 34(1): 173–186.
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