Affiliation:
1. Azerbaijan Medical University
Abstract
PURPOSE. To create a model of uveitis in conditions of experimental glaucoma and to study various immunological blood parameters.METHODS. The experiment was conducted on 24 sexually mature Chinchilla rabbits, divided into 2 groups. In group I (16 eyes), steroid glaucoma was modeled. To create the model, the animals were instilled with 1 drop of 0.1% dexamethasone 2 times a day for 30 days. In group II, sensitization with normal horse serum (NHS) was performed in addition to experimental steroid glaucoma modeling (16 rabbits). To create a model of uveitis, a resolving dose of NHS was injected into the right eye of the animals of group II (16 eyes). The right eye (16 eyes) constituted subgroup 1, the left eye (16 eyes) — subgroup 2 (control). Blood was taken from the ear vein of all animals. Sample 1 consisted of specimens from animals in group I, samples 2 and 3 — specimens from animals in group II after sensitization and after uveitis development, respectively.RESULTS. Clinical picture of uveitis was observed in the right eye 3 days after injecting the resolving intravitreal dose. The following changes were noted in samples 2 and 3, respectively: the leukocyte content increased by 95.9% (p<0.001) and 90.8% (p<0.001); the neutrophil content decreased by 22% (p=0.417) and increased by 105.8% (p<0.001), total hemolytic complement capacity decreased by 84.4% (p<0.001) and 84.3% (p<0.001), the number of circulating immune complexes decreased by 99.1% (p<0.001) and 96.9% (p<0.001), the number of T-lymphocytes increased by 120.5% (p<0.001) and 116.8% (p<0.001), and B-lymphocytes — by 93.0% (p<0.001) in both samples, IgE — by 5.3 times (p<0.001) and 6.5 times (p<0.001).CONCLUSION. The obtained model of uveitis in conditions of experimental glaucoma will allow a more detailed study of the important links of the pathological process in the eye, and to extrapolate the obtained data to clinical practice in order to improve the effectiveness and safety of pathogenetically oriented treatment.
Publisher
Research Institute of Eye Diseases
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