Author:
KANAGAWA H.,FRIM J.,KRUUV J.
Abstract
The zona pellucida of fertilized bovine embryos of the morula stage (day 6) were punctured using a micromanipulator and a glass knife. In the presence of 2 M DMSO, 23 embryos were cooled (0.5 °C/min) to −196 °C and, upon thawing (10 °C/min), transferred into nine recipients. Although the embryos, before and after the freeze-thaw cycle, were morphologically indistinguishable, only one calf was born. The role of the zona pellucida during freezing and thawing is discussed.
Publisher
Canadian Science Publishing
Subject
Animal Science and Zoology,Food Animals
Cited by
18 articles.
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