Preparation of rabbit polyclonal antibodies against lupin storage proteins

Abstract

Agarose gel electrophoresis was used to separate three globular storage proteins from crude extracts of Lupinus albus seeds. Conglutin α, β, and γ purified by this technique were used as antigens to stimulate the production of antibodies in rabbits. The specificity of polyclonal antibodies produced was analysed by Western blot. Each antibody reacted with the specific antigen used to stimulate production of that antibody. In addition the anti-conglutin α preparation also showed binding to a region of the gel that comigrates with conglutin β. Key words: Lupin, conglutin, polyclonal antibody