Affiliation:
1. Agriculture and Agri-Food Canada, Lacombe Research Centre, 6000 C & E Trail Lacombe, Alberta, Canada T4L 1W1
2. Instituto de Ganadería de Montaña (Consejo Superior de Investigaciones Científicas, Universidad de León), Finca Marzanas, E-24346 Grulleros, León, Spain
Abstract
Turner, T., Rolland, D. C., Aldai, N. and Dugan, M. E. R. 2011. Short Communication: Rapid separation of cis 9, trans 11- and trans 7,c is 9-18:2 (CLA) isomers from ruminant tissue using a 30 m SLB-IL111 ionic column. Can. J. Anim. Sci. 91: 711–713. Rumenic acid (cis9,trans11-18:2) is the main natural isomer of conjugated linoleic acid (CLA). Rumenic acid has many purported health benefits, but effects of most other CLA isomers are unknown. Typically trans7,cis9-18:2 is the second most abundant CLA isomer, but it co-elutes with rumenic acid on conventional polar gas chromatography (GC) columns, requiring complimentary analysis with silver-ion high performance liquid chromatography (Ag+-HPLC). Herein we report a rapid method for analyzing rumenic acid and trans7,cis9-18:2 using a 30 m ionic-liquid GC column. Optimal resolution of the two CLA isomers was at 145°C and analysis of backfat from barley-fed cattle compared well with GC/Ag+-HPLC (y=0.978x – 0.031, r=0.985, P<0.001).
Publisher
Canadian Science Publishing
Subject
Animal Science and Zoology,Food Animals
Reference12 articles.
1. Canadian Council on Animal Care. 1993. Guide to the care and use of experimental animals. Vol 1, 2nd ed. E. E. Olfert, B. M. Cross, and A. A. McWilliams, eds. Canadian Council on Animal Care, Ottawa, ON. 212pp.
2. Relative retention order of all isomers of cis/trans conjugated linoleic acid FAME from the 6,8- to 13,15-positions using silver ion HPLC with two elution systems
3. Separation characteristics of fatty acid methyl esters using SLB-IL111, a new ionic liquid coated capillary gas chromatographic column
4. Combining Results of Two GC Separations Partly Achieves Determination of All cis and trans 16:1, 18:1, 18:2 and 18:3 Except CLA Isomers of Milk Fat as Demonstrated Using Ag-Ion SPE Fractionation
Cited by
23 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献