Abstract
Optimal assay conditions for porcine hepatic and adipose stearoyl-CoA desaturase systems were established. In each of the two experiments conducted, pigs were allotted to four groups in a 2 × 2 factorial design involving two diets (basal or basal plus 250 ppm supplemental copper) and two sexes (barrows and gilts). In experiment 1 (16 pigs), copper supplementation of the swine diet resulted in a slight but nonsignificant increase in the ability of hepatic microsomes to desaturate 1-14C-stearoyl-CoA. With an increased number of animals (24 pigs) in experiment 2, significant increases in stearoyl-CoA desaturase activity were observed in the hepatic (P < 0.05) and adipose (P < 0.01) microsomes of copper-supplemented pigs. No sex differences nor copper × sex interactions in the activities of the stearoyl-CoA desaturase systems were found. It is suggested that supplemental dietary copper enhances the specific activities of both hepatic and adipose stearoyl-CoA desaturase systems, and that this enhancement contributes to the observed decrease in stearate and increase in oleate content of depot fat.
Publisher
Canadian Science Publishing
Subject
Animal Science and Zoology,Food Animals
Cited by
30 articles.
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